The only requirement for your visa/ residence permit to be used as supporting visa is that it should be still valid (timewise) by the time you enter Turkey. Used single entry visas are also accepted as long as their validity date covers your entry date to Turkey. Please note that e-Visas of other countries are not accepted as supporting visas.
https://www.evisa.gov.tr/en/info/
官网写了 使用过的单次申根等visa可以作为入境的依据,只要申根等visa在入境土耳其的时刻仍然在有效期内。
所以,使用过没问题,但必须在有效期内。